Reverse genetics identifies the product of open reading frame 4 as an essential particle assembly factor of Nyamanini virus.
نویسندگان
چکیده
We established a reverse genetics system for Nyamanini virus (NYMV) and recovered green fluorescent protein (GFP)-expressing virus from full-length cDNA. Using this technology, we assessed the functions of two poorly characterized viral genes. NYMV lacking open reading frame 2 (ORF2) could not be rescued, whereas virus lacking ORF4 was replication competent. ORF4-deficient NYMV readily established a persisting noncytolytic infection but failed to produce infectious viral particles, supporting the view that ORF4 represents an essential factor for NYMV particle assembly.
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ورودعنوان ژورنال:
- Journal of virology
دوره 87 14 شماره
صفحات -
تاریخ انتشار 2013